Products

MilliTrace constitutive GFP reporter neural stem cell lines

Millipore Corporation, recently introduced their MilliTrace primary rodent neural stem cell (NSC) lines that express green fluorescent protein (GFP) constitutively. GFP expression in these stem cells is the best way for researchers to monitor the behavior of specific populations of cells as they proliferate, migrate, and differentiate into various cell lineages, depending on developmental context. The MilliTrace cell lines are the first commercially available, GFP-expressing, karyotypically normal stem cell lines, and are supplied with optimised expansion medium. Many immortal cell lines display unstable or aneuploid chromosomes; however, for stem cell lines to yield physiologically relevant results, it is important that the cells display normal chromosomes. Unlike human NSC cultures, rodent NSCs undergo prolonged self-renewal in culture without losing chromosomal stability. Researchers at Millipore have been able to isolate and propagate monolayer cultures of NSCs from two different regions of the rodent brain the adult rat hippocampus and the embryonic mouse cortex. Even after introduction of constitutively expressing GFP, the NSCs from these tissues show normal karyotype, stability over more than 10 passages, and multipotency. Both cell lines can be readily differentiated into neurons, astrocytes, or oligodendrocytes, using appropriate cues.

Validated for high levels of GFP expression, stem cell marker expression, and multipotency, MilliTrace GFP Reporter Neural Stem Cell Lines can improve reproducibility and data quality for a variety of applications. Researchers can use MilliTrace cell lines to study cell-cell interactions in co-culture studies or contribution of NSCs to the stem cell niche in vivo. MilliTrace cell lines also enable high-throughput screens to discover agents that affect stem cell maintenance and differentiation.

Fast Membrane Filtration of high particulate and viscous solutions

Millipore Corporation, recently informed about expansion of its Millex HPF line of syringe filters, which improve throughput of high particulate and viscous solutions. Notably, Millex HPF filters are now available with a new hydrophilic teflon membrane, which exhibits the broadest chemical compatibility and lowest extractability of any membrane. When used to filter samples and liquid buffers, the new Millex filters improve the quality and consistency of results, sample throughput, and the longevity of expensive analytical equipment.

The Millex HPF filter incorporates multiple media in its design a graduated glass fiber pre-filter and a membrane filter. This multi-layer filter is resistant to clogging by viscous and high particulate solutions, yet provides quantitative retention of fine particulates. Hydrophilic teflon and nylon Millex HPF filters are now available with 0.2 µm and 0.45 µm pore sizes, meeting the needs of researchers filtering samples and mobile phases for Ultra High Pressure Liquid Chromatography (UHPLC), a very sensitive and increasingly popular separation technique.

Given their low analyte binding and low extractable characteristics, Millex HPF filters are ideal for a number of applications, including drug dissolution testing, wine analysis, and sample preparation for chromatography. The new Millex filters are specifically engineered for automatic filter-changing workstations, commonly used in high throughput sample preparation. The domed filter housing ensures reliable filter delivery, minimising workstation downtime.

PureProteome nickel magnetic beads

Millipore Corporation's, PureProteome Nickel Magnetic Beads will be now available in the market. This powerful separation matrix allows the fast and easy purification of polyhistidine-tagged recombinant proteins, a critical step in protein research. Millipore's patented process for magnetising porous silica particles provides researchers with high binding capacity, high affinity beads for efficient protein purification. Unlike many competitors' magnetic beads, PureProteome Nickel Magnetic beads bind recombinant protein even in the presence of EDTA, a metal ion chelator. Compared to other magnetic beads, Millipore's beads offer higher purity while yielding comparable amounts of protein.

The integrated Millipore offers more innovative technologies and stronger application support to streamline processes and provide consistently reliable results. Their laboratory filtration portfolio includes thousands of membrane-based filtration tools for use in the laboratory to purify and separate samples prior to analysis to allow researchers to efficiently achieve consistent, high quality results. Millipore has evolved from a high performance filtration products and services company into a leading provider of tools and services and a key partner of choice for Life Science customers. The integrated Millipore offers more innovative technologies and stronger application support to streamline processes and provide consistently reliable results.

Contact details:
Millipore India Pvt. Ltd.
50 A, II Phase, Ring Road, Peenya,
Bangalore - 560058
Tel nos: 080 39224000, 39224001
Fax: 080 39286345
Contact person: Krishna Kumar
Email id: krishna_kumar@milliporeindia.com
Website: www.millipore.com

Mixing Battery

Industrial Equip Wash mixing battery is designed to provide instantaneous hot water economically by mixing steam and cold water to the required temperature through the simple turn of the Knob. The mixing battery is supplied with isolation valves, check valves, union joints, strainers and temperature indicating gauge to know the temperature of the emerging hot water.

Mixing battery provides industry with cheap hot water where ever steam is available along with the water.

It incorporates hydraulic fail safe device so that when correctly installed and maintained, no steam regardless of its pressure can enter the mixing chamber until the water flows to raise the steam valve of its seat. When turned off at the outlet, there is immediate and positive shut-off.

The unit can be provided with self rewinding hose reel. Using heavy duty internal spring the reel quickly and easily rewinds the hose.

The ratchets assembly locks the reel when the desigred length of the hose is pulled out. All hose reel are preset with enough force to fully retract the hose. The hose reel is fabricated from SS-304/SS-306 for heavy duty use.

Contact details:
Industrial Equipwash
8/B, Surat Singh Industrial Estate,
Behind Agrawal Industrial Estate,
S V Road, Jogeshwari (West),
Mumbai - 400 102.
Tel: 022 26797941 / 26798066
Fax: 022 26792936 / 26798066
Mobile: 9869231815
Email: iewi@vsnl.net
Website: www.iewi.net